Plasmid

Part:BBa_I714946:Design

Designed by: Xili Liu   Group: iGEM07_Peking   (2007-10-25)


pCC002


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 210
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 1251
    Illegal EcoRI site found at 2718
    Illegal XbaI site found at 247
    Illegal XbaI site found at 1282
    Illegal SpeI site found at 268
    Illegal SpeI site found at 2134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 210
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 1251
    Illegal EcoRI site found at 2718
    Illegal SpeI site found at 268
    Illegal SpeI site found at 2134
    Illegal NotI site found at 236
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 210
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 1251
    Illegal EcoRI site found at 2718
    Illegal BamHI site found at 224
    Illegal XhoI site found at 281
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 210
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 1251
    Illegal EcoRI site found at 2718
    Illegal XbaI site found at 247
    Illegal XbaI site found at 1282
    Illegal SpeI site found at 268
    Illegal SpeI site found at 2134
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 210
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 1251
    Illegal EcoRI site found at 2718
    Illegal XbaI site found at 247
    Illegal XbaI site found at 1282
    Illegal SpeI site found at 268
    Illegal SpeI site found at 2134
    Illegal AgeI site found at 704
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

to measure the efficiency of different SD signal, the plasmid has a MCS downstream of Ptet where can insert reporters along with SD signal. There is a T1 terminator downstream of MCS to terminate transcription starting from Ptet. Ptet is repressed by TetR carried on this plasmid and can be turned on by adding aTc.


Source

pCRT-1

References